DTD1 Recombinant monoclonal antibody

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  WB analysis using 87297-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87297-1-RR (DTD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 87297-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DTD1 antibody (87297-1-RR, Clone: 252568A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DTD1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87297-1-RR

Clone No.252568A10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

C20orf88, D-aminoacyl-tRNA deacylase 1, DNA-unwinding element-binding protein B, DTD, DUEB

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, U2OS cells, mouse testis tissue, rat testis tissue, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87297-1-RR targets DTD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11510

  Product name: Recombinant human DTD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-209 aa of BC045167

  Sequence: MKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDKQYEILCVSQFTLQCVLKGNKPDFNLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGPVTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP

• Full Name: D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 209 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC045167
• Gene Symbol: DTD1
• Gene ID (NCBI): 92675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TEA8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DTD1 (D-aminoacyl-tRNA deacylase 1) is a crucial enzyme that ensures the fidelity of protein synthesis by maintaining L-homochirality. It functions as a proofreading factor that hydrolyzes mischarged D-aminoacyl-tRNAs into free D-amino acids and tRNA molecules, preventing the incorporation of D-amino acids into polypeptide chains. DTD1 also plays a significant part in the initiation of DNA replication by facilitating the loading of the CDC45 complex onto pre-replication sites. (PMID: 18700836;27200345)