DTYMK Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86543-3-RR (same clone as 86543-3-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 86543-3-RR (DTYMK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86543-3-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 86543-3-RR (same clone as 86543-3-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 86543-3-RR (DTYMK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86543-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86543-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86543-3-RR against Human DTYMK were performed. The affinity constant is 0.565 nM.

DTYMK Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86543-3-PBS

Clone No.251441C1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

CDC8, dTMP kinase, EC:2.7.4.9, TMPK, TYMK

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Product Information

86543-3-PBS targets DTYMK in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7606

  Product name: Recombinant human DTYMK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-212 aa of BC001827

  Sequence: MAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK

• Full Name: deoxythymidylate kinase (thymidylate kinase)
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC001827
• Gene Symbol: DTYMK
• Gene ID (NCBI): 1841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23919
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DTYMK(Thymidylate kinase) is also named as CDC8,TMPK and TYMK.It catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.This enzyme is important in cellular DNA synthesis because the synthesis of dTTP either via the de novo pathway or through the exogenous supply of thymidine requires the activity of this enzyme(PMID:18469).