DTYMK Recombinant monoclonal antibody

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  WB analysis using 86543-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 86543-3-RR (DTYMK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 86543-3-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 86543-3-RR (DTYMK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86543-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86543-3-RR against Human DTYMK were performed. The affinity constant is 0.565 nM.

DTYMK Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86543-3-RR

Clone No.251441C1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

CDC8, dTMP kinase, EC:2.7.4.9, TMPK, TYMK

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Tested Applications

• Positive WB detected in: HeLa cells, rat brain tissue, HepG2 cells, Raji cells, MOLT-4 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86543-3-RR targets DTYMK in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7606

  Product name: Recombinant human DTYMK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-212 aa of BC001827

  Sequence: MAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK

• Full Name: deoxythymidylate kinase (thymidylate kinase)
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC001827
• Gene Symbol: DTYMK
• Gene ID (NCBI): 1841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23919
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DTYMK(Thymidylate kinase) is also named as CDC8,TMPK and TYMK.It catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.This enzyme is important in cellular DNA synthesis because the synthesis of dTTP either via the de novo pathway or through the exogenous supply of thymidine requires the activity of this enzyme(PMID:18469).