DUOX1 Monoclonal antibody

DUOX1 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

3B8D6

Cat no : 67226-1-Ig

Synonyms

dual oxidase 1, DUOX, DUOX1, Large NOX 1, LNOX1, Long NOX 1, NADPH thyroid oxidase 1, NOXEF1, THOX1, Thyroid oxidase 1

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Validation Data Gallery

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  WB analysis of human testis using 67226-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67226-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67226-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse stomach using 67226-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using DUOX1 antibody (67226-1-Ig, Clone: 3B8D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human testis tissue, human placenta tissue
• Positive IF-P detected in: mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

67226-1-Ig targets DUOX1 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: DUOX1 fusion protein Ag18154
• Full Name: dual oxidase 1
• Calculated Molecular Weight: 1551 aa, 177 kDa
• Observed Molecular Weight: 190-200 kDa, 177 kDa, 138 kDa
• GenBank Accession Number: BC114938
• Gene Symbol: DUOX1
• Gene ID (NCBI): 53905
• RRID: AB_2882515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUOX1 (dual oxidase 1) is a calciumactivated transmembrane protein member of the NOX family of NADPH oxidases principally located on the apical surface of airway and thyroid epithelia (PMID: 28982074, 15677770, 10806195). DUOX1 is a strong generator of hydrogen peroxide (H2O2), via intra-molecular dismutation of superoxide (PMID: 25586178). Epithelial DUOX1 appears to participate in additional host defense actions, such as mucociliary clearance, and may contribute to inflammatory responses and epithelial repair processes during exogenous stress (PMID: 19386603). DUOX1 has two isofroms with the calculated molecular mass of 177 and 138 kDa, and apparent molecular mass of 180-200 kDa due to the glycosylation (PMID: 17643428, 19144650).

Publications

Species	Application	Title
human	IF	Cancers (Basel); Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.; Authors - Luc H J Sondorp
human	WB	BMC Mol Cell Biol; Upregulated dual oxidase 1-induced oxidative stress and caspase-1-dependent pyroptosis reflect the etiologies of heart failure; Authors - Yan Song Li