DUS4L Polyclonal antibody

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  WB analysis of mouse kidney using 21870-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21870-1-AP (DUS4L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 21870-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21870-1-AP (DUS4L Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21870-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21870-1-AP (DUS4L Antibody) at dilution of 1:200 (under 40x lens).

DUS4L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21870-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

DUS4, DUS4L, PP35

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21870-1-AP targets DUS4L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16145

  Product name: Recombinant human DUS4L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-197 aa of BC111774

  Sequence: AMAEGYGACLINKPELVQDMVKQVRNQVETPGFSVSIKIRIHDDLKRTVDLCQKAEATGVSWITVHGRTAEERHQPVHYDSIKIIKENMSIPVIANGDIRSLKEAENVWRITGTDGVMVARGLLANPAMFAGYEETPLKCIWDWVDIALELGTPYMCFHQHLMYMMEKITSRQEKRVFNALSSTSAIIDYLTDHYGI

• Full Name: dihydrouridine synthase 4-like (S. cerevisiae)
• Calculated Molecular Weight: 317 aa, 36 kDa
• Observed Molecular Weight: 22 kDa, 30 kDa
• GenBank Accession Number: BC111774
• Gene Symbol: DUS4L
• Gene ID (NCBI): 11062
• RRID: AB_2878933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95620
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

tRNA-dihydrouridine synthase 4-like belongs to the dus family.It catalyzes the synthesis of dihydrouridine and a modified base found in the D-loop of most tRNAs.Researches showed that DUS4L was alsophosphorylated in vitro by a Ca2+ calmodulin-activated kinase. It has 2 isoforms produced by alternative splicing .One, at 35 kDa, comigrated with a second cAMP-stimulated RIS-ROS phosphoprotein,DUS4L,which was also detected in the cytoskeleton.This antibody recognize the C-terminal of DUS4L.