DUSP6 Polyclonal antibody, PBS Only

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• DUSP6 Polyclonal antibody, PBS Only (26071-1-PBS)

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  WB analysis using 26071-1-AP (same clone as 26071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26071-1-AP (DUSP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-12 using 26071-1-AP (same clone as 26071-1-PBS)

  IP result of anti-DUSP6 (IP:26071-1-AP, 4ug; Detection:26071-1-AP 1:1000) with PC-12 cells lysate 1040 ug. This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 26071-1-AP (same clone as 26071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 26071-1-AP (DUSP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 26071-1-AP (same clone as 26071-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DUSP6 antibody (26071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 26071-1-AP (same clone as 26071-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using DUSP6 antibody (26071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 26071-1-AP (same clone as 26071-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUSP6 Polyclonal antibody (26071-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

DUSP6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 26071-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

MKP3, MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16

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Product Information

26071-1-PBS targets DUSP6 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23474

  Product name: Recombinant human DUSP6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 353-381 aa of BC003562

  Sequence: CDNRVPAQQLYFTTPSNQNVYQVDSLQST

• Full Name: dual specificity phosphatase 6
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC003562
• Gene Symbol: DUSP6
• Gene ID (NCBI): 1848
• RRID: AB_3669521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16828
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).