DUSP6 Recombinant monoclonal antibody

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• DUSP6 Recombinant monoclonal antibody (83356-5-RR)

DUSP6 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83356-5-RR Copied!

Clone No. 240295C8

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

240295C8, dual specificity phosphatase 6, EC:3.1.3.16, MAP kinase phosphatase 3, Mitogen-activated protein kinase phosphatase 3

• 

  WB analysis using 83356-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83356-5-RR (DUSP6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 83356-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of PC-12 using 83356-5-RR

  1x10^6 PC-12 cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 83356-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83356-5-RR against Human DUSP6 were performed. The affinity constant is 3.38 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, HepG2 cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue
• Positive FC (Intra) detected in: HeLa cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83356-5-RR targets DUSP6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag23474

  Product name: Recombinant human DUSP6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 353-381 aa of BC003562

  Sequence: CDNRVPAQQLYFTTPSNQNVYQVDSLQST

• Full Name: dual specificity phosphatase 6
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC003562
• Gene Symbol: DUSP6
• Gene ID (NCBI): 1848
• RRID: AB_3671013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16828
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product is a 5-star rating. The reviewer used a 1:100 dilution on human brain cortex tissue for immunofluorescence and reported that DUSP6 (visualized in green) worked very well in human FFPE brain tissue. Overall, the feedback is positive, highlighting strong performance in FFPE tissue staining.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Reyes (Verified Customer) (09-30-2025)

DUSP6 (in green) worked very well in human FFPE brain tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

DUSP6 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:83356-5-RR)