Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 cells using 12037-1-AP (same clone as 12037-1-PBS)
WB result of DVL2 antibody (12037-1-AP, 1:1000) with siDVL2 and si-control transfected cells. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
WB analysis using 12037-1-AP (same clone as 12037-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
WB analysis of mouse thymus using 12037-1-AP (same clone as 12037-1-PBS)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 12037-1-AP (same clone as 12037-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
WB analysis of MDA-MB-453s using 12037-1-AP (same clone as 12037-1-PBS)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IP experiment of MCF-7 using 12037-1-AP (same clone as 12037-1-PBS)
IP result of anti-DVL2 (IP:12037-1-AP, 4ug; Detection:12037-1-AP 1:4000) with MCF-7 cells lysate 640 ug. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 12037-1-AP (same clone as 12037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12037-1-AP (same clone as 12037-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DVL2 antibody (12037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

DVL2 Polyclonal antibody, PBS Only

DVL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12037-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

12037-1-PBS targets DVL2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2666 Product name: Recombinant human DVL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC014844 Sequence: MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDI Predict reactive species
Full Name	dishevelled, dsh homolog 2 (Drosophila)
Calculated Molecular Weight	736 aa, 79 kDa
Observed Molecular Weight	90-95 kDa
GenBank Accession Number	BC014844
Gene Symbol	DVL2
Gene ID (NCBI)	1856
RRID	AB_2093330
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14641
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes.