ChromoTek DYKDDDDK Fab-Trap™ Agarose

The ChromoTek DYKDDDDK Fab-Trap™ Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Specificity

Flag®-tag (1x and 3x)

Applications

IP, CoIP, ChIP, RIP, Protein purification

Type

Fab-fragment

Conjugate

Agarose

Cat no : ffa

Synonyms

Fab-Trap Agarose, Fab-Trap, DYKDDDDK, flag



Product Information

The ChromoTek DYKDDDDK Fab-Trap™ Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

DescriptionThe DYKDDDDK Fab-Trap Agarose is a premium affinity resin for efficient immunoprecipitation (IP) of Flag®-tagged proteins. The DYKDDDDK Fab-Trap Agarose consists of a Fab-fragment, which is covalently bound to agarose beads.

• Lowest background in class

• Less contaminating antibody chains

• Recombinantly expressed Fab-fragment derived from M2 clone

• Compatible with harsh buffer conditions

• Ready-to-use

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetThe DYKDDDDK Fab-Trap™ Agarose specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). It binds to same epitope as Sigma's Anti-FLAG® M2 Antibody and is compatible with N-, C-terminal or internal tagging.
Binding capacity12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer3xDYKDDDDK-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility0.5 % Nonidet P40 Substitute, 5 % Tween™ 20, 2 M NaCl, 10 mM TCEP, 10 mM DTT, 10 mM ß-mercaptoethanol, 2 M Urea
Type Fab-fragment
ClassRecombinant
HostMouse
RRIDAB_2894836
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

mBio

The Cajal body protein p80-coilin forms a complex with the adenovirus L4-22K protein and facilitates the nuclear export of adenovirus mRNA

Authors - Laura White
CoIP

Life Sci Alliance

RAS and PP2A activities converge on epigenetic gene regulation

Authors - Anna Aakula
IP

bioRxiv

Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins

Authors - Alaina H Willet

Sci Adv

Codependencies of mTORC1 signaling and endolysosomal actin structures

Authors - Amulya Priya

Mol Biol Cell

Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins

Authors - Alaina H Willet

Biophys J

Characterization of human XPD helicase activity with Single Molecule Magnetic Tweezers

Authors - Chunfeng Mao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hana (Verified Customer) (06-08-2023)

In this immunoprecipitation experiment, I have tested your DYKDDDDK Fab-Trap™ Agarose (ffa) beads, which are very nicely and effectively pulling down FLAG-tagged MCMBP protein. MCMBP is a strong interactor of MCMs. In the attached image, we have pulled down the MCM7 and MCM5 subunits as we previously described in Sedlackova et. al., Nature, 2020. Additionally in this experiment, we tested the FLAG antibody (80010-1-RR, dilution for western blot was 1:1000, 5%milk in 1xPBS+0,1% Tween, incubated overnight) and MCM5 antibody (11703-1-AP, dilution for western blot was 1:1000, 5%milk in 1xPBS+0,1% Tween, incubated overnight). All your products worked amazingly well.

  • Applications: Western Blot,Immunoprecipitation
  • Cell Tissue Type: human osteosarcoma U2OS
FH

Jonasz (Verified Customer) (11-15-2022)

The ffa-2 DYKDDDDK Fab-Trap agarose fulfilled our expection and showed excellent performance comparable to other Trap products by Chromotek/Proteintech such as GFP-Trap or myc-Trap agarose. We successfully precipitated FLAG-tagged ubiquitin overexpressed in HEK293T cells.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HEK 293T cells
FH

Abida (Verified Customer) (11-02-2022)

Stain-free gel (A) and western blot (B) of immunoprecipitation of flag-fusion protein from Lactococcus lactis bacterial cell lysate with DYKDDDDK Fab trap agarose beads (Proteintech, cat no ffa). Flag-fusion protein was eluted from DYKDDDDK Fab-Trap with 2x SDS-sample buffer. Western blot was probed with rabbit DYKDDDDK tag polyclonal antibody (Proteintech, cat no 20543-1-AP) followed by anti-rabbit StarBright IgG Blue 520 fluorescent goat secondary antibody (BioRad, cat no 12005869). I: input (lysate), M: protein marker, FT: flowthrought, B: bound (elution).

  • Applications: Immunoprecipitation
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Bacteria Lactococcus lactis
FH

Tobias (Verified Customer) (10-25-2022)

excellent efficiency and yield.

  • Applications: Immunoprecipitation
FH

Peter (Verified Customer) (10-24-2022)

Excellent recovery of flag tagged proteins with short incubation times

  • Applications: Immunoprecipitation
  • Cell Tissue Type: A549
FH

Amit (Verified Customer) (03-16-2022)

Works well for purification of FLAG tag protein purification and immunoprecipitation. Highly recommended!

  • Applications: Immunoprecipitation,Protein purification
  • Cell Tissue Type: E.Coli