DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

8H6A10

Cat no : 66008-4-PBS

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag

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Validation Data Gallery

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  WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

  HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

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  WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

  HEK-293 and DYKDDDDK-TDP43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

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  WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

  HEK-293 and DYKDDDDK-PCSK9 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDKtag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

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  IP experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

  IP result of anti-DYKDDDDK tag (IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed DYKDDDDK-TDP43 Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66008-4-PBS targets DYKDDDDK tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DYKDDDDK tag
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.