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DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope) (66008-4-PBS)

DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope)

Name: DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope), 100ug
Brand: Proteintech
SKU: 66008-4-PBS-100UG

DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 66008-4-PBS

Clone No. 8H6A10

Publications (1)

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Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBIPIF/ICCFC (Intra)

WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)
HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.
WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)
HEK-293 and DYKDDDDK-TDP43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)
HEK-293 and DYKDDDDK-PCSK9 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDKtag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
HEK-293 and DYKDDDDK-PCSK9 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDKtag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.
IP experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)
IP result of anti-DYKDDDDK tag (IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3). This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)
1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-4-Ig, Clone:8H6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-4-Ig, Clone:8H6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

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Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Product Information

66008-4-PBS targets DYKDDDDK tag in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity	recombinant protein
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	DYKDDDDK tag
Gene Symbol
Gene ID (NCBI)
RRID	AB_2918475
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	FLAGTAG
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publications

What published studies show

The DYKDDDDK tag Monoclonal antibody (8H6A10), catalog number 66008-4-PBS, has 1 indexed citation. It appears in Biochimica et Biophysica Acta – Molecular Basis of Disease (2026). The associated research field is cardiology, specifically studying myocardial ischemia-reperfusion injury and the role of ubiquitin-specific protease 11 and YY1 in regulating voltage-dependent anion channel 1 during myocardial infarction.

All (1)

Species	Application	Title
		Biochim Biophys Acta Mol Basis Dis Ubiquitin-specific protease 11 and YY1 mediate myocardial ischemia-reperfusion injury in myocardial infarction by regulating the ubiquitination and transcription of voltage-dependent anion channel 1. Authors - Wei Zheng View Article