DeltaN p63 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86154-11-RR (same clone as 86154-11-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

• 

  WB analysis using 86154-11-RR (same clone as 86154-11-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

• 

  WB analysis using 86154-11-RR (same clone as 86154-11-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (DeltaN p63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 86154-11-RR (same clone as 86154-11-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TP63 DeltaN(P40) antibody (86154-11-RR, Clone: 251209D11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

DeltaN p63 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86154-11-PBS

Clone No.251209D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DeltaNp63 alpha, p40 - DeltaNp63, p40/deltaNp63, ΔNp63, p63

---

Product Information

86154-11-PBS targets DeltaN p63 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor protein p63
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: NM_001114980
• Gene Symbol: TP63
• Gene ID (NCBI): 8626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H3D4-2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DeltaN p63 (ΔNp63) is a major protein isoform encoded by the TP63 gene. The TP63 gene is a member of the p53 gene family, which also includes p53 and p73. The key defining feature of DeltaN p63 (ΔNp63) is the absence of the full N-terminal transactivation domain. However, it retains the central DNA-binding domain (DBD) and the C-terminal oligomerization domain (OD). The α-isoform also has a sterile alpha motif (SAM) domain and a transcriptional inhibitory domain (TID). DeltaN p63 isoforms can act as dominant-negative inhibitors of p53 and TAp63 by competing for DNA binding sites or forming non-functional dimers. DeltaN p63 is highly expressed in basal layers of epithelial tissues and is essential for the maintenance and proliferation of epithelial stem cells. It plays a pivotal role in epidermal development, stratification, and regeneration.