DeltaN p63 Recombinant monoclonal antibody

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  WB analysis using 86154-11-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86154-11-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86154-11-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (DeltaN p63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 86154-11-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TP63 DeltaN(P40) antibody (86154-11-RR, Clone: 251209D11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

DeltaN p63 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86154-11-RR

Clone No.251209D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DeltaNp63 alpha, p40 - DeltaNp63, p40/deltaNp63, ΔNp63, p63

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Tested Applications

• Positive WB detected in: MCF-7 cells, A431 cells, HaCaT cells, U-937 cells, PC-12 cells, mouse skin tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86154-11-RR targets DeltaN p63 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor protein p63
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: NM_001114980
• Gene Symbol: TP63
• Gene ID (NCBI): 8626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H3D4-2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DeltaN p63 (ΔNp63) is a major protein isoform encoded by the TP63 gene. The TP63 gene is a member of the p53 gene family, which also includes p53 and p73. The key defining feature of DeltaN p63 (ΔNp63) is the absence of the full N-terminal transactivation domain. However, it retains the central DNA-binding domain (DBD) and the C-terminal oligomerization domain (OD). The α-isoform also has a sterile alpha motif (SAM) domain and a transcriptional inhibitory domain (TID). DeltaN p63 isoforms can act as dominant-negative inhibitors of p53 and TAp63 by competing for DNA binding sites or forming non-functional dimers. DeltaN p63 is highly expressed in basal layers of epithelial tissues and is essential for the maintenance and proliferation of epithelial stem cells. It plays a pivotal role in epidermal development, stratification, and regeneration.