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CoraLite®594-conjugated Desmin Monoclonal antibody

Desmin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B1A11

Cat no : CL594-67793

Synonyms

CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using CoraLite®594-conjugated Desmin Monoclonal antibody (CL594-67793)

    IF Staining of human colon using CL594-67793

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 Desmin antibody (CL594-67793, Clone: 2B1A11 ) at dilution of 1:200. DAPI (blue).

Tested Applications

Positive IF detected inhuman colon tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67793 targets Desmin in IF applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Desmin fusion protein Ag9675
Full Name desmin
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC032116
Gene Symbol DES
Gene ID (NCBI) 1674
RRIDAB_2920184
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocols

Product Specific Protocols
IF protocol for CL594 Desmin antibody CL594-67793Download protocol
Standard Protocols
Click here to view our Standard Protocols