Destrin Monoclonal antibody

Destrin Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1A12D9

Cat no : 67657-1-Ig

Synonyms

ACTDP, Actin depolymerizing factor, ADF, bA462D18.2, Destrin, DSN, DSTN

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Validation Data Gallery

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  WB analysis using 67657-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67657-1-Ig (Destrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, human platelet, pig rectum, rat rectum, pig brain, rat brain, mouse brain

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67657-1-Ig targets Destrin in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Destrin fusion protein Ag1402
• Full Name: destrin (actin depolymerizing factor)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-19 kDa
• GenBank Accession Number: BC009477
• Gene Symbol: Destrin
• Gene ID (NCBI): 11034
• RRID: AB_2918496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis; Authors - Xiaoguang Liu