Di-Methyl-Histone H3 (Lys56) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82828-12-RR (same clone as 82828-12-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82828-12-RR (HIST1H3A(H3K56me2) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 82828-12-RR (same clone as 82828-12-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Lys56) antibody (82828-12-RR, Clone: 243027A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82828-12-RR (same clone as 82828-12-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Di-Methyl-Histone H3 (Lys56) antibody (82828-12-RR, Clone: 243027A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

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  Dot Blot experiment using 82828-12-RR (same clone as 82828-12-PBS)

  Dot blot analysis was used to confirm the specificity of 82828-12-RR (Di-Methyl-Histone H3 (Lys56) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

Di-Methyl-Histone H3 (Lys56) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA

Cat No. 82828-12-PBS

Clone No.243027A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA

Synonyms

Di Methyl Histone H3, Di-Methyl Histone H3 (Lys56), Di-Methyl Histone H3 Lys56, Di-Methyl Histone-H3 Lys56, Di-Methyl-Histone H3

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Product Information

82828-12-PBS targets Di-Methyl-Histone H3 (Lys56) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15-18 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).