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Tested Applications
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-86990-2 targets Di-Methyl-Histone H3 (Lys9) in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | histone cluster 1, H3a |
| Observed Molecular Weight | 15-17 kDa |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
H3K9me2 is a typical transcriptional repressive modification, primarily enriched in heterochromatin regions (such as centromeres, telomeres, and transposon elements), but can also be scattered throughout euchromatin. By recruiting "reader" proteins such as HP1 (Heterochromatin Protein 1), H3K9me2 can establish and stabilize a chromatin condensation state, thereby silencing gene expression.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Di-Methyl-Histone H3 (Lys9) antibody CL488-86990-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
