Dystroglycan Polyclonal antibody, PBS Only

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• Dystroglycan Polyclonal antibody, PBS Only (11017-1-PBS)

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  WB analysis using 11017-1-AP (same clone as 11017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 11017-1-AP (same clone as 11017-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

  IP result of anti-Dystroglycan (IP:11017-1-AP, 4ug; Detection:11017-1-AP 1:500) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11017-1-AP (same clone as 11017-1-PBS)

  Immunofluorescent analysis of HeLa cells using 11017-1-AP (Dystroglycan antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 11017-1-AP (same clone as 11017-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Dystroglycan (11017-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Dystroglycan Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11017-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

DAG1, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG

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Product Information

11017-1-PBS targets Dystroglycan in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1456

  Product name: Recombinant human DAG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 546-895 aa of BC012740

  Sequence: VGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDRAPARFKAKFVGDPALVLNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQIAGLSRRIAEDDGKPRPAFSNALEPDFKATSITVTGSGSCRHLQFIPVVPPRRVPSEAPPTEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPLNQDTMGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

• Full Name: dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC012740
• Gene Symbol: Dystroglycan
• Gene ID (NCBI): 1605
• RRID: AB_2090032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14118
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).