FcZero-rAb™ Biotin Anti-Human E-cadherin Rabbit Recombinant Antibody

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  FC experiment of HT-29 using Biotin-FcA98030

  1x10^6 HT-29 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Biotin Anti-Human E-cadherin Rabbit RecAb (Biotin-FcA98030, Clone:240159G8) and Streptavidin-PE Conjugate (PE-PF00030) (red), or 5 ul FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

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  FC experiment of MCF-7 using Biotin-FcA98030

  1x10^6 MCF-7 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Biotin Anti-Human E-cadherin Rabbit RecAb (Biotin-FcA98030, Clone:240159G8) and Streptavidin-PE Conjugate (PE-PF00030) (red), or 5 ul FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

E-cadherin FcZero-rAb^TM Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98030

Clone No.240159G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

240159G8, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

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Tested Applications

• Positive FC detected in: HT-29 cells, MCF-7 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98030 targets E-cadherin in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14973

  Product name: Recombinant human E-cadherin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 373-622 aa of BC141838

  Sequence: PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPI

• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 882 aa, 97 kDa
• GenBank Accession Number: BC141838
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 999
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12830
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.