CoraLite®594 Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody

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  FC experiment of HUVEC using CL594-98189

  1x10^6 untreated or TNF alpha-treated HUVEC cells were surface stained with 5 ul CoraLite®594 Anti-Human E-selectin/CD62E Rabbit RecAb (CL594-98189, Clone: 241767H6). Cells were not fixed.

• 

  FC experiment of HUVEC using CL594-98189

  1x10^6 TNF alpha-treated HUVEC cells were surface stained with 5 ul CoraLite®594 Anti-Human E-selectin/CD62E Rabbit RecAb (CL594-98189, Clone: 241767H6) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated HUVEC cells were surface stained with 5 ul CoraLite®594 Anti-Human E-selectin/CD62E Rabbit RecAb (CL594-98189, Clone: 241767H6) (black, dashed). Cells were not fixed.

E-selectin/CD62E Uni-rAb^® Recombinant Antibody for FC

Cat No. CL594-98189

Clone No.241767H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD62E, E-selectin, 241767H6, CD62 antigen-like family member E, E selectin

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Tested Applications

• Positive FC detected in: TNF alpha treated HUVEC cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98189 targets E-selectin/CD62E in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: selectin E
• Calculated Molecular Weight: 610 aa, 67 kDa
• GenBank Accession Number: BC142711
• Gene Symbol: CD62E
• Gene ID (NCBI): 6401
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16581
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)