FcZero-rAb® PE Anti-Mouse E-selectin/CD62E Rabbit Recombinant Antibody

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• FcZero-rAb® PE Anti-Mouse E-selectin/CD62E Rabbit Recombinant Antibody (PE-FcA98065)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

E-selectin/CD62E FcZero-rAb^® Recombinant Antibody for FC

Cat No. PE-FcA98065 Copied!

Clone No. 240700D4

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD62E, E-selectin, E selectin, Elam, Sele

• 

  FC experiment of bEnd.3 using PE-FcA98065

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug PE Anti-Mouse E-selectin/CD62E Rabbit RecAb (PE-FcA98065, Clone: 240700D4) (red), or 0.25 ug PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: bEnd.3 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98065 targets E-selectin/CD62E in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0610

  Product name: Recombinant mouse E-selectin protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 22-557 aa of NM_011345.2

  Sequence: WYYNASSELMTYDEASAYCQRDYTHLVAIQNKEEINYLNSNLKHSPSYYWIGIRKVNNVWIWVGTGKPLTEEAQNWAPGEPNNKQRNEDCVEIYIQRTKDSGMWNDERCNKKKLALCYTASCTNASCSGHGECIETINSYTCKCHPGFLGPNCEQAVTCKPQEHPDYGSLNCSHPFGPFSYNSSCSFGCKRGYLPSSMETTVRCTSSGEWSAPAPACHVVECEALTHPAHGIRKCSSNPGSYPWNTTCTFDCVEGYRRVGAQNLQCTSSGIWDNETPSCKAVTCDAIPQPQNGFVSCSHSTAGELAFKSSCNFTCEQSFTLQGPAQVECSAQGQWTPQIPVCKAVQCEALSAPQQGNMKCLPSASGPFQNGSSCEFSCEEGFELKGSRRLQCGPRGEWDSKKPTCSAVKCDDVPRPQNGVMECAHATTGEFTYKSSCAFQCNEGFSLHGSAQLECTSQGKWTQEVPSCQVVQCPSLDVPGKMNMSCSGTAVFGTVCEFTCPDDWTLNGSAVLTCGATGRWSGMPPTCEAPVSPTRP

• Full Name: selectin, endothelial cell
• Calculated Molecular Weight: 67KD
• GenBank Accession Number: NM_011345.2
• Gene Symbol: Sele
• Gene ID (NCBI): 20339
• RRID: AB_3749728
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00690
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)