E2F8 Polyclonal antibody

E2F8 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13425-1-AP

Synonyms

E2F 8, E2F transcription factor 8, E2F8, Transcription factor E2F8

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Validation Data Gallery

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  WB analysis of HeLa using 13425-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13425-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13425-1-AP

  IP result of anti-E2F8 (IP:13425-1-AP, 4ug; Detection:13425-1-AP 1:500) with HEK-293 cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• ChIP: See 2 publications below

Product Information

13425-1-AP targets E2F8 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E2F8 fusion protein Ag4216
• Full Name: E2F transcription factor 8
• Calculated Molecular Weight: 867 aa, 94 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC028244
• Gene Symbol: E2F8
• Gene ID (NCBI): 79733
• RRID: AB_2097273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)

Publications

Species	Application	Title
human	WB	Int J Cancer; HOXD-AS1/miR-130a sponge regulates glioma development by targeting E2F8.; Authors - Yinan Chen
human	WB,ChIP	Adv Sci (Weinh); Genetic Aberrations and Interaction of NEK2 and TP53 Accelerate Aggressiveness of Multiple Myeloma; Authors - Xiangling Feng
human	ChIP	Int J Toxicol; Transcription Factor E2F8 Promotes Cisplatin Resistance in Hepatocellular Carcinoma by Regulating DNA Damage via NUSAP1; Authors - Jianqiao Kong
human	WB	Cell Death Discov; Carmofur prevents cell cycle progression by reducing E2F8 transcription in temozolomide-resistant glioblastoma cells; Authors - Cyntanna C Hawkins