EAAT3 Polyclonal antibody, PBS Only

Name: EAAT3 Polyclonal antibody, PBS Only,
Brand: Proteintech

EAAT3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12686-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

SLC1A1, EAAT 3, Excitatory amino-acid carrier 1, HEAAC1, Slc1 a1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

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Filter: AllWBIHC

WB analysis of mouse ovary using 12686-1-AP (same clone as 12686-1-PBS)
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse ovary using 12686-1-AP (same clone as 12686-1-PBS)
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 12686-1-AP (same clone as 12686-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 12686-1-AP (same clone as 12686-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 12686-1-AP (same clone as 12686-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 12686-1-AP (same clone as 12686-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 12686-1-AP (same clone as 12686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 12686-1-AP (same clone as 12686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 12686-1-AP (same clone as 12686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 12686-1-AP (same clone as 12686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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Product Information

12686-1-PBS targets EAAT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3379 Product name: Recombinant human SLC1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-524 aa of BC033040 Sequence: AEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF Predict reactive species
Full Name	solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Calculated Molecular Weight	524 aa, 57 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC033040
Gene Symbol	EAAT3
Gene ID (NCBI)	6505
RRID	AB_2188001
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43005
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

EAAT3 (Excitatory Amino Acid Transporter 3), encoded by human SLC1A1 (Gene ID: 6505), is a neuronal high-affinity glutamate transporter of the SLC1 family. It is expressed mainly in CNS neurons, and also in intestinal and renal epithelial cells. EAAT3 is the only EAAT subtype that efficiently transports L-cysteine, supplying substrate for neuronal glutathione (GSH) synthesis and antioxidant defense. Its transport cycle couples uptake of L-glutamate, L-aspartate, or L-cysteine to 3 Na⁺/1 H⁺ co-transport and 1 K⁺ counter-transport. EAAT3 also contributes to metabolic reprogramming in activated B cells and cancer cells, and can transport the oncometabolite R-2-hydroxyglutarate (R-2HG). SLC1A1 mutations are linked to dicarboxylic aminoaciduria and schizophrenia susceptibility.