EBF1 Polyclonal antibody

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  WB analysis of U2OS using 14159-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14159-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of 3T3-L1 using 14159-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 14159-1-AP (EBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

EBF1 Polyclonal Antibody for WB, ELISA

Cat No. 14159-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

COE1, Early B-cell factor, EBF, O/E-1, OE-1

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Tested Applications

• Positive WB detected in: U2OS cells, Raji cells, 3T3-L1 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14159-1-AP targets EBF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5341

  Product name: Recombinant human EBF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 4-350 aa of BC038805

  Sequence: IQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP

• Full Name: early B-cell factor 1
• Calculated Molecular Weight: 591 aa, 64 kDa
• Observed Molecular Weight: 57-67 kDa
• GenBank Accession Number: BC038805
• Gene Symbol: EBF1
• Gene ID (NCBI): 1879
• RRID: AB_2231057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UH73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EBF1, also named as O/E-1, OE-1, OLF1, COE1 and EBF, belongs to the COE family. It is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. EBF1 is a key factor for the specification of B-cell lineage.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB	iScience; Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9; Authors - Ting Wang
mouse	WB	ACS Nano; Arginine Supplementation Targeting Tumor-Killing Immune Cells Reconstructs the Tumor Microenvironment and Enhances the Antitumor Immune Response; Authors - Junlei Zhang