|Positive WB detected in||U2OS cells, Raji cells, 3T3-L1 cells|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14159-1-AP targets EBF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EBF1 fusion protein Ag5341|
|Full Name||early B-cell factor 1|
|Calculated molecular weight||591 aa, 64 kDa|
|Observed molecular weight||57-67 kDa|
|GenBank accession number||BC038805|
|Gene ID (NCBI)||1879|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EBF1, also named as O/E-1, OE-1, OLF1, COE1 and EBF, belongs to the COE family. It is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. EBF1 is a key factor for the specification of B-cell lineage.
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Arginine Supplementation Targeting Tumor-Killing Immune Cells Reconstructs the Tumor Microenvironment and Enhances the Antitumor Immune Response