EBP Polyclonal antibody

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  WB analysis using 12968-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12968-1-AP (EBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 12968-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12968-1-AP (EBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12968-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12968-1-AP (EBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EBP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12968-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CPX, Cholestenol Delta-isomerase, Cholestenol Delta isomerase, CHO2, CDPX2

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, mouse liver tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12968-1-AP targets EBP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3630

  Product name: Recombinant human EBP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-230 aa of BC001572

  Sequence: MTTNAGPLHPYWPQHLRLDNFVPNDRPTWHILAGLFSVTGVLVVTTWLLSGRAAVVPLGTWRRLSLCWFAVCGFIHLVIEGWFVLYYEDLLGDQAFLSQLWKEYAKGDSRYILGDNFTVCMETITACLWGPLSLWVVIAFLRQHPLRFILQLVVSVGQIYGDVLYFLTEHRDGFQHGELGHPLYFWFYFVFMNALWLVLPGVLVLDAVKHLTHAQSTLDAKATKAKSKKN

• Full Name: emopamil binding protein (sterol isomerase)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC001572
• Gene Symbol: EBP
• Gene ID (NCBI): 10682
• RRID: AB_3085403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15125
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.