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EBP50/NHERF-1 Polyclonal antibody, PBS Only (29771-1-PBS)

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 29771-1-AP (same clone as 29771-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 29771-1-AP (same clone as 29771-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 29771-1-AP (same clone as 29771-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 29771-1-AP (same clone as 29771-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 (29771-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.
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1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 (29771-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

EBP50/NHERF-1 Polyclonal antibody, PBS Only

Name: EBP50/NHERF-1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 29771-1-PBS

EBP50/NHERF-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 29771-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

SLC9A3R1, NHERF-1, EBP50

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

29771-1-PBS targets EBP50/NHERF-1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag30878 Product name: Recombinant human SLC9A3R1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 260-358 aa of NM_004252 Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL Predict reactive species
Full Name	solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	NM_004252
Gene Symbol	SLC9A3R1/EBP50/NHERF-1
Gene ID (NCBI)	9368
RRID	AB_2935478
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14745
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).