ECD Monoclonal antibody, PBS Only

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  WB analysis using 67179-1-Ig (same clone as 67179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67179-1-Ig (ECD antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse bladder using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse bladder using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat bladder using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat bladder using 67179-1-Ig (same clone as 67179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67179-1-PBS in a different storage buffer formulation.

ECD Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67179-1-PBS

Clone No.1D5E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2

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Product Information

67179-1-PBS targets ECD in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28674

  Product name: Recombinant human ECD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 378-641 aa of BC000721

  Sequence: LAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKP

• Full Name: ecdysoneless homolog (Drosophila)
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70-73 kDa
• GenBank Accession Number: BC000721
• Gene Symbol: ECD
• Gene ID (NCBI): 11319
• RRID: AB_2882475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O95905
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.