ECD Monoclonal antibody

ECD Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67179-1-Ig
Clone No.1D5E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, JAR cells, NCCIT cells, HEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human stomach cancer tissue, mouse bladder tissue, rat colon tissue, rat bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67179-1-Ig targets ECD in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ECD fusion protein Ag28674 Predict reactive species
Full Name ecdysoneless homolog (Drosophila)
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70-73 kDa
GenBank Accession NumberBC000721
Gene Symbol ECD
Gene ID (NCBI) 11319
RRIDAB_2882475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95905
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ECD antibody 67179-1-IgDownload protocol
IHC protocol for ECD antibody 67179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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