Filter: AllWBIHC

WB analysis using 67179-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67179-1-Ig (ECD antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
IHC staining of human renal cell carcinoma using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat bladder using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat bladder using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse bladder using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse bladder using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 67179-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ECD Monoclonal antibody

Name: ECD Monoclonal antibody
Brand: Proteintech
SKU: 67179-1-Ig

ECD Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67179-1-Ig

Clone No.1D5E8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, JAR cells, NCCIT cells, HEK-293 cells
Positive IHC detected in	human renal cell carcinoma tissue, human stomach cancer tissue, mouse bladder tissue, rat colon tissue, rat bladder tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67179-1-Ig targets ECD in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	ECD fusion protein Ag28674 Predict reactive species
Full Name	ecdysoneless homolog (Drosophila)
Calculated Molecular Weight	73 kDa
Observed Molecular Weight	70-73 kDa
GenBank Accession Number	BC000721
Gene Symbol	ECD
Gene ID (NCBI)	11319
RRID	AB_2882475
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O95905
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.