EDC3 Polyclonal antibody

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• EDC3 Polyclonal antibody (16486-1-AP)

EDC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16486-1-AP Copied!

Publications (8)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

YJDC, PP844, LSM16 homolog, LSM16, hYjeF_N2

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  WB analysis using 16486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16486-1-AP (EDC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 16486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16486-1-AP (EDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HeLa using 16486-1-AP

  IP result of anti-EDC3 (IP:16486-1-AP, 4ug; Detection:16486-1-AP 1:500) with HeLa cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human kidney using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16486-1-AP (EDC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16486-1-AP (EDC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 16486-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EDC3 antibody (16486-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293T cells, MCF-7 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human heart tissue, human placenta tissue, human skin tissue, human liver tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16486-1-AP targets EDC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9608

  Product name: Recombinant human EDC3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 158-508 aa of BC021271

  Sequence: RHNSWSSSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEGNLALFDKAAVFEEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVSKEFCTDSGLVVPSISYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQGISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDCPENVFLRDQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGEHAGRIYLCDIGIPQQVFQEVGINYHSPFGCKFVIPLHSA

• Full Name: enhancer of mRNA decapping 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 508 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC021271
• Gene Symbol: EDC3
• Gene ID (NCBI): 80153
• RRID: AB_2095946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96F86
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The EDC3 Polyclonal antibody (Cat# 16486-1-AP) has 8 citations across journals including Cancer Research, Neuron, Nucleic Acids Research, Cell Reports, Journal of Cell Biology, EMBO Reports, PLoS Genetics, and bioRxiv. Research fields span cancer biology (EMT, glioblastoma), neurodegeneration (TDP-43), RNA processing (P-bodies, stress granules, DDX6), cardiomyocyte regulation, and meiotic germ cell biology.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
human	IF	Neuron; DCPS modulates TDP-43-linked neurodegeneration through P-body-mediated RNA decay.; Authors - Yingzhi Ye
human		Nucleic Acids Res; RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules; Authors - Vladimir Majerciak
rat	WB	Cell Rep; Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.; Authors - Eva Riechert
human	IF,WB	J Cell Biol; DDX6 modulates P-body and stress granule assembly, composition, and docking; Authors - Nina Ripin
human	WB	EMBO Rep; Praja2 controls P-body assembly and translation in glioblastoma by non-proteolytic ubiquitylation of DDX6; Authors - Emanuela Senatore

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single available review for this product is highly positive, with a 5-star rating. The reviewer used the antibody for immunohistochemistry and reported excellent performance, specifically noting it worked great with ECL (enhanced chemiluminescence) detection. Overall, the feedback indicates strong reliability and satisfactory results in the tested application.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Aditya (Verified Customer) (11-01-2024)

great with ecl

• Applications: Immunohistochemistry