Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17737-1-AP targets EDC4 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11784 Product name: Recombinant human EDC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-388 aa of BC064567 Sequence: MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMPPINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAVSNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVNGKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWPVDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSCLLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSW Predict reactive species |
| Full Name | enhancer of mRNA decapping 4 |
| Calculated Molecular Weight | 1401 aa, 152 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC064567 |
| Gene Symbol | EDC4 |
| Gene ID (NCBI) | 23644 |
| RRID | AB_10665813 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P2E9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Enhancer of mRNA-decapping protein 4 (EDC4) is a key regulator of mRNA decapping and degradation, playing a crucial role in the mRNA decay pathway. It is involved in the formation of processing bodies (P-bodies) in the cytoplasm, where it interacts with other decapping factors such as DCP1A and DCP2A to facilitate mRNA degradation (PMID: 16364915). EDC4 also interacts with the mammalian target of rapamycin complex 1 (mTORC1) and is involved in the regulation of immune system functions (PMID: 25514416; 25970328). Additionally, EDC4 has been shown to regulate DNA repair processes, with its deficiency leading to genome instability and hypersensitivity to DNA interstrand cross-linking drugs (PMID: 29511213).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EDC4 antibody 17737-1-AP | Download protocol |
| IHC protocol for EDC4 antibody 17737-1-AP | Download protocol |
| WB protocol for EDC4 antibody 17737-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EDC4 Polyclonal antibody (Cat# 17737-1-AP) has 21 citations spanning journals including Nature Cell Biology, Nature Communications, Cancer Research, Cell Discovery, Science Immunology, Oncogene, Genes & Development, and FEBS Letters. Research fields cover P-body and stress granule biology, mRNA decapping, phase separation, cancer (EMT, chemosensitivity, bone metastasis), centrosome/ciliogenesis, oocyte maturation, Ebola virus infection, and inflammasome activation.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
Cancer Res Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition | ||
Nat Commun Systematic characterization of the composition and dynamics of processing body-associated mRNAs. | ||
Nat Commun A protein-proximity screen reveals Ebola virus co-opts the mRNA decapping complex through the scaffold protein EDC4.
| ||
Cell Discov RNA helicase DDX6 governs ASC speck formation in P-bodies and the transition to stress granules via phase separation during inflammasome activation. | ||
Sci Immunol DDX6 induces immunosuppression in cancer by disrupting structural stability of endogenous double-stranded RNAs. |
Reviews
What customers say
Both reviews rate the antibody 5 stars. One user describes it as a favorite for IHC, used at a 1:400 dilution. The other used it at 1:200 for immunofluorescence on human RPE-1 cells, co-staining with EDC4 and NEDD4 markers. The protocol involved 4% PFA fixation, 0.3% Triton permeabilization, and 5% BSA blocking, with the primary antibody incubated for 1 hour at room temperature. Overall, reviewers report strong performance across Western Blot, IHC, and IF applications, with clear staining and reliable results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (09-02-2026) | favorite antibody for IHC
|
Elisa (Verified Customer) (03-01-2022) | RPE1 cells stained for Hoechst (DNA marker, in green), EDC4 (mRNA decapping protein 4 in (P)-bodies, in magenta) and NEDD1 (pericentriolar matrix marker, in yellow). RPE1 cells were fixed in 4%PFA for 15’. Cells were then washed with PBS. Membrane permeabilization was then performed with 0.3% Triton for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
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