CoraLite® Plus 488-conjugated EDC4 Polyclonal antibody

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  IF Staining of HeLa using CL488-17737

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EDC4 antibody (CL488-17737) at dilution of 1:400, CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

EDC4 Polyclonal Antibody for IF/ICC

Cat No. CL488-17737

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Autoantigen Ge 1, Autoantigen RCD 8, EDC4, enhancer of mRNA decapping 4, Ge 1, HEDLS, RCD 8

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17737 targets EDC4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11784

  Product name: Recombinant human EDC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-388 aa of BC064567

  Sequence: MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMPPINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAVSNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVNGKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWPVDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSCLLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSW

• Full Name: enhancer of mRNA decapping 4
• Calculated Molecular Weight: 1401 aa, 152 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC064567
• Gene Symbol: EDC4
• Gene ID (NCBI): 23644
• RRID: AB_3084007
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P2E9
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Enhancer of mRNA-decapping protein 4 (EDC4) is a key regulator of mRNA decapping and degradation, playing a crucial role in the mRNA decay pathway. It is involved in the formation of processing bodies (P-bodies) in the cytoplasm, where it interacts with other decapping factors such as DCP1A and DCP2A to facilitate mRNA degradation (PMID: 16364915). EDC4 also interacts with the mammalian target of rapamycin complex 1 (mTORC1) and is involved in the regulation of immune system functions (PMID: 25514416; 25970328). Additionally, EDC4 has been shown to regulate DNA repair processes, with its deficiency leading to genome instability and hypersensitivity to DNA interstrand cross-linking drugs (PMID: 29511213).