Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-17737 targets EDC4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11784 Product name: Recombinant human EDC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-388 aa of BC064567 Sequence: MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMPPINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAVSNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVNGKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWPVDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSCLLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSW Predict reactive species |
| Full Name | enhancer of mRNA decapping 4 |
| Calculated Molecular Weight | 1401 aa, 152 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC064567 |
| Gene Symbol | EDC4 |
| Gene ID (NCBI) | 23644 |
| RRID | AB_3084007 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P2E9 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Enhancer of mRNA-decapping protein 4 (EDC4) is a key regulator of mRNA decapping and degradation, playing a crucial role in the mRNA decay pathway. It is involved in the formation of processing bodies (P-bodies) in the cytoplasm, where it interacts with other decapping factors such as DCP1A and DCP2A to facilitate mRNA degradation (PMID: 16364915). EDC4 also interacts with the mammalian target of rapamycin complex 1 (mTORC1) and is involved in the regulation of immune system functions (PMID: 25514416; 25970328). Additionally, EDC4 has been shown to regulate DNA repair processes, with its deficiency leading to genome instability and hypersensitivity to DNA interstrand cross-linking drugs (PMID: 29511213).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EDC4 antibody CL488-17737 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EDC4 Polyclonal antibody (CL488-17737) has 1 citation. It appears in Cell Reports (2025), in a study on YTHDF1 phase separation and tumorigenesis. The research field encompasses RNA metabolism, phase separation, and cancer biology. The antibody was used for immunofluorescence in human samples.
| Species | Application | Title |
|---|---|---|
