EDF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EDF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12419-1-AP

Synonyms

EDF 1, EDF1, MBF1, Multiprotein bridging factor 1

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Validation Data Gallery

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  WB analysis of A549 using 12419-1-AP

  WB result of EDF1 antibody (12419-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-EDF1 transfected A549 cells.

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  WB analysis of Jurkat using 12419-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12419-1-AP (EDF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12419-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12419-1-AP (EDF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12419-1-AP

  IP result of anti-EDF1 (IP:12419-1-AP, 4ug; Detection:12419-1-AP 1:500) with Jurkat cells lysate 2920 ug.

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  IHC staining of human prostate cancer using 12419-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12419-1-AP (EDF1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells, mouse pancreas tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below

Product Information

12419-1-AP targets EDF1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EDF1 fusion protein Ag3063
• Full Name: endothelial differentiation-related factor 1
• Calculated Molecular Weight: 148 aa, 16 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC015500
• Gene Symbol: EDF1
• Gene ID (NCBI): 8721
• RRID: AB_2230895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IP	Nat Commun; Long noncoding RNA licensing of obesity-linked hepatic lipogenesis and NAFLD pathogenesis.; Authors - Xu-Yun Zhao
human	WB	Front Neurol; Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.; Authors - Matthias Höllerhage
mouse	WB	bioRxiv; Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletion; Authors - Shiyu Luo
mouse	WB	Dis Model Mech; HBS1L deficiency causes retinal dystrophy in a child and a mouse model associated with defective development of photoreceptor cells; Authors - Shiyu Luo