Endothelin 1 Polyclonal antibody

Endothelin 1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12191-1-AP

Synonyms

EDN1, endothelin 1, ET 1 Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inrat lung tissue, mouse lung tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12191-1-AP targets Endothelin 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Endothelin 1 fusion protein Ag2847
Full Name endothelin 1
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC009720
Gene symbol EDN1
Gene ID (NCBI) 1906
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).

Protocols

Product Specific Protocols
WB protocol for Endothelin 1 antibody 12191-1-APDownload protocol
IHC protocol for Endothelin 1 antibody 12191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy

Authors - Lijie Ma
humanWB, IP

Cancer Res

Lactoferrin-endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes.

Authors - Ngoc-Han Ha
mouseIF

Redox Biol

Blocking mitochondrial cyclophilin D ameliorates TSH-impaired defensive barrier of artery.

Authors - Xiaojing Liu
humanWB

Int J Biol Sci

Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.

Authors - Meicen Zhou
humanWB

Cancer Gene Ther

Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor.

Authors - Chae Young Hwang
  • KD Validated
humanWB

J Cell Mol Med

Macrophage-derived exosomal miR-4532 promotes endothelial cells injury by targeting SP1 and NF-κB P65 signalling activation

Authors - Peng Liu