Endothelin 1 Polyclonal antibody

Endothelin 1 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 12191-1-AP

Synonyms

EDN1, endothelin 1, ET 1 Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 12191-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12191-1-AP (Endothelin 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 12191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12191-1-AP (Endothelin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 12191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12191-1-AP (Endothelin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HUVEC using 12191-1-AP

  1X10^6 HUVEC cells were intracellularly stained with 0.4 ug Anti-Human Endothelin 1 (12191-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: mouse lung tissue, mouse placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12191-1-AP targets Endothelin 1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Endothelin 1 fusion protein Ag2847
• Full Name: endothelin 1
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC009720
• Gene Symbol: EDN1
• Gene ID (NCBI): 1906
• RRID: AB_889392
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).

Publications

Species	Application	Title
mouse	WB	Redox Biol; Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy; Authors - Lijie Ma
human	WB, IP	Cancer Res; Lactoferrin-endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes.; Authors - Ngoc-Han Ha
mouse	IF	Redox Biol; Blocking mitochondrial cyclophilin D ameliorates TSH-impaired defensive barrier of artery.; Authors - Xiaojing Liu
human	WB	Int J Biol Sci; Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.; Authors - Meicen Zhou
human	WB	Cancer Gene Ther; Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor.; Authors - Chae Young Hwang ; KD Validated
human	WB	J Cell Mol Med; Macrophage-derived exosomal miR-4532 promotes endothelial cells injury by targeting SP1 and NF-κB P65 signalling activation; Authors - Peng Liu

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