Endothelin 1 Polyclonal antibody

Endothelin 1 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


mouse and More (2)





Cat no : 12191-1-AP


EDN1, endothelin 1, ET 1 Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1

Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inmouse lung tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12191-1-AP targets Endothelin 1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Endothelin 1 fusion protein Ag2847
Full Name endothelin 1
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC009720
Gene Symbol EDN1
Gene ID (NCBI) 1906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).


Product Specific Protocols
WB protocol for Endothelin 1 antibody 12191-1-APDownload protocol
IHC protocol for Endothelin 1 antibody 12191-1-APDownload protocol
FC protocol for Endothelin 1 antibody 12191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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