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Endothelin 1 Polyclonal antibody
Endothelin 1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 12191-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NIH/3T3 cells |
Positive IHC detected in | mouse lung tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HUVEC cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 19 publications below |
IHC | See 3 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Product Information
12191-1-AP targets Endothelin 1 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Endothelin 1 fusion protein Ag2847 |
Full Name | endothelin 1 |
Calculated Molecular Weight | 212 aa, 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC009720 |
Gene Symbol | Endothelin 1 |
Gene ID (NCBI) | 1906 |
RRID | AB_889392 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).
Protocols
Product Specific Protocols | |
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WB protocol for Endothelin 1 antibody 12191-1-AP | Download protocol |
IHC protocol for Endothelin 1 antibody 12191-1-AP | Download protocol |
FC protocol for Endothelin 1 antibody 12191-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Redox Biol Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy | ||
Cancer Res Lactoferrin-endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes. | ||
Redox Biol Blocking mitochondrial cyclophilin D ameliorates TSH-impaired defensive barrier of artery. | ||
Int J Biol Sci Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA. | ||
Cancer Gene Ther Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor.
| ||
J Cell Mol Med Macrophage-derived exosomal miR-4532 promotes endothelial cells injury by targeting SP1 and NF-κB P65 signalling activation |