Endothelin 1 Monoclonal antibody

Endothelin 1 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C5B3

Cat no : 67008-1-Ig

Synonyms

EDN1, endothelin 1, ET 1 Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1



Tested Applications

Positive WB detected inhuman placenta tissue
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67008-1-Ig targets Endothelin 1 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Endothelin 1 fusion protein Ag28334
Full Name endothelin 1
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC009720
Gene Symbol EDN1
Gene ID (NCBI) 1906
RRIDAB_2882325
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).

Protocols

Product Specific Protocols
WB protocol for Endothelin 1 antibody 67008-1-IgDownload protocol
FC protocol for Endothelin 1 antibody 67008-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Rep

Cilostazol regulates the expressions of endothelin-1 and endothelial nitric oxide synthase via activation of the p38 MAPK signaling pathway in HUVECs

Authors - Ying Xue
humanWB

Hypertens Res

Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation

Authors - Zheng Wang