CoraLite® Plus 750-conjugated EDNRB Recombinant monoclonal antibody

• 

  WB analysis using CL750-82818-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82818-2 (EDNRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

EDNRB Uni-rAb^® Recombinant Antibody for WB

Cat No. CL750-82818-2

Clone No.1O2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Synonyms

ET B, endothelin receptor type B, Endothelin receptor non-selective type, Endothelin B receptor, ABCDS

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82818-2 targets EDNRB in WB applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: endothelin receptor type B
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_000115
• Gene Symbol: EDNRB
• Gene ID (NCBI): 1910
• RRID: AB_3673742
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24530
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).