|Positive IF detected in||HeLa cells|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66218-1-Ig targets EEA1 in WB, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgM|
|Full Name||early endosome antigen 1|
|Calculated molecular weight||162 kDa|
|GenBank accession number||NM_003566|
|Gene ID (NCBI)||8411|
|Purification Method||Thiophilic affinity chromatograph|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-16-2021)
Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cell line that was pre-treated by the drug of interest and labelled by EEA1 monoclonal antibody at dilution of 1:50 showed strong labelling of early endosomes.