EED Polyclonal antibody, PBS Only

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• EED Polyclonal antibody, PBS Only (16818-1-PBS)

• 

  WB analysis of COLO 320 using 16818-1-AP (same clone as 16818-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16818-1-AP (same clone as 16818-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 16818-1-AP (same clone as 16818-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 16818-1-AP (same clone as 16818-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 16818-1-AP (same clone as 16818-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 16818-1-AP (same clone as 16818-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 16818-1-AP (same clone as 16818-1-PBS)

  IP result of anti-EED (IP:16818-1-AP, 4ug; Detection:16818-1-AP 1:500) with PC-3 cells lysate 3200ug. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16818-1-AP (same clone as 16818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16818-1-AP (same clone as 16818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

  Immunofluorescent analysis of (10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16818-1-AP (same clone as 16818-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EED (16818-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

EED Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 16818-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

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Product Information

16818-1-PBS targets EED in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10358

  Product name: Recombinant human EED protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-466 aa of BC068995

  Sequence: MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSGRAILHSHQQCMRDPVSPNLRQHLSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR

• Full Name: embryonic ectoderm development
• Calculated Molecular Weight: 466 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC068995
• Gene Symbol: EED
• Gene ID (NCBI): 8726
• RRID: AB_2262065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75530
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.