Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67495-1-Ig targets EEF1A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1938 Product name: Recombinant human EEF1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 144-462 aa of BC014224 Sequence: GVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK Predict reactive species |
| Full Name | eukaryotic translation elongation factor 1 alpha 1 |
| Calculated Molecular Weight | 462 aa, 50 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC014224 |
| Gene Symbol | EEF1A1 |
| Gene ID (NCBI) | 1915 |
| RRID | AB_2882719 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P68104 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for EEF1A1 antibody 67495-1-Ig | Download protocol |
| IF protocol for EEF1A1 antibody 67495-1-Ig | Download protocol |
| IHC protocol for EEF1A1 antibody 67495-1-Ig | Download protocol |
| WB protocol for EEF1A1 antibody 67495-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EEF1A1 Monoclonal antibody (1A9F5), catalog number 67495-1-Ig, has 7 total citations. These appear in Cell Chem Biol, Cell Reports, EMBO Reports, Biology Direct, STAR Protocols, Chem Biol Drug Des, and Molecular Cell. The associated research fields span mRNA translation and translational control, cGAMP-binding protein identification, hepatocellular carcinoma, glioblastoma, and renal cell carcinoma progression.
| Species | Application | Title |
|---|---|---|
Cell Chem Biol A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP. | ||
Cell Rep Coordinated regulation of mRNA translation and stability by ZC3H7A and ZC3H7B RNA-binding proteins. | ||
Biol Direct UCHL3 promotes hepatocellular carcinoma progression by stabilizing EEF1A1 through deubiquitination | ||
STAR Protoc Protocol for identification and validation of 2'3'-cGAMP-binding proteins by photoaffinity probes. | ||
Mol Cell PHYHD1-mediated translational control regulates renal cell carcinoma progression. |
