EEF1A1 Monoclonal antibody

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  WB analysis of HepG2 using 67495-1-Ig

  WB result of EEF1A1 antibody (67495-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HepG2 cells.

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  WB analysis using 67495-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67495-1-Ig (EEF1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67495-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67495-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67495-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67495-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67495-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67495-1-Ig (EEF1A1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 67495-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EEF1A1 (67495-1-Ig, Clone:1A9F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

EEF1A1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 67495-1-Ig

Clone No.1A9F5

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

EEF1A, EEF 1, EC:3.6.5.-, CCS3, CCS 3

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human pancreas cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

67495-1-Ig targets EEF1A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EEF1A1 fusion protein Ag1938
• Full Name: eukaryotic translation elongation factor 1 alpha 1
• Calculated Molecular Weight: 462 aa, 50 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014224
• Gene Symbol: EEF1A1
• Gene ID (NCBI): 1915
• RRID: AB_2882719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68104
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou
human	WB,IP	STAR Protoc; Protocol for identification and validation of 2'3'-cGAMP-binding proteins by photoaffinity probes.; Authors - Yingjie Hou
human	IF	Biol Direct; UCHL3 promotes hepatocellular carcinoma progression by stabilizing EEF1A1 through deubiquitination; Authors - Jie Zhao