|Positive WB detected in||SKOV-3 cells, HEK-293 cells, PC-3 cells, HeLa cells, RAW264.7 , Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10095-2-AP targets EEF1B2 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EEF1B2 fusion protein Ag0135|
|Full Name||eukaryotic translation elongation factor 1 beta 2|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||30-34 kDa|
|GenBank accession number||BC000211|
|Gene ID (NCBI)||1933|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.
|Product Specific Protocols|
|WB protocol for EEF1B2 antibody 10095-2-AP||Download protocol|
|IHC protocol for EEF1B2 antibody 10095-2-AP||Download protocol|
|IF protocol for EEF1B2 antibody 10095-2-AP||Download protocol|
|IP protocol for EEF1B2 antibody 10095-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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