• Featured Product
  • KD/KO Validated

EEF1B2 Polyclonal antibody

EEF1B2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10095-2-AP

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta



Tested Applications

Positive WB detected inSKOV-3 cells, HEK-293 cells, PC-3 cells, HeLa cells, RAW264.7 , Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10095-2-AP targets EEF1B2 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1B2 fusion protein Ag0135
Full Name eukaryotic translation elongation factor 1 beta 2
Calculated molecular weight 25 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC000211
Gene symbol EEF1B2
Gene ID (NCBI) 1933
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.

Protocols

Product Specific Protocols
WB protocol for EEF1B2 antibody 10095-2-APDownload protocol
IHC protocol for EEF1B2 antibody 10095-2-APDownload protocol
IF protocol for EEF1B2 antibody 10095-2-APDownload protocol
IP protocol for EEF1B2 antibody 10095-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biotechniques

En masse analysis of nascent translation using microarrays.

Authors - Mazan-Mamczarz Krystyna K
mouse, humanWB, IHC, IF

PLoS One

Characterisation of Translation Elongation Factor eEF1B Subunit Expression in Mammalian Cells and Tissues and Co-Localisation with eEF1A2.

Authors - Yuan Cao
  • KD Validated
humanWB

Front Microbiol

Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins.

Authors - Shuhei Sammaibashi
humanWB,IF,IP

Mol Cell Biol

Translational repression by RNA-binding protein TIAR.

Authors - Mazan-Mamczarz Krystyna K
RIP

Nat Cell Biol

The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing PXN expression through the alternative splicing of lncRNA-PXN-AS1.

Authors - Ji-Hang Yuan