EEF1D Monoclonal antibody, PBS Only

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  WB analysis of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60085-2-Ig (same clone as 60085-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60085-2-Ig (same clone as 60085-2-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60085-2-Ig (same clone as 60085-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60085-2-Ig (same clone as 60085-2-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

  IP result of anti-EEF1D (IP:60085-2-Ig, 5ug; Detection:60085-2-Ig 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 60085-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 60085-2-Ig against Human EEF1D were performed. The Affinity Constant is 0.76 nM.

• Featured Product
• KD/KO Validated

EEF1D Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 60085-2-PBS

Clone No.3G4C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

3G4C4, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D

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Product Information

60085-2-PBS targets EEF1D in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0983

  Product name: Recombinant human EEF1D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 347-647 aa of BC007847

  Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

• Full Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC007847
• Gene Symbol: EEF1D
• Gene ID (NCBI): 1936
• RRID: AB_2096994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29692
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )