EEF2 Polyclonal antibody

EEF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20107-1-AP

Synonyms

EEF 2, EEF2, EF 2, EF2, Elongation factor 2



Tested Applications

Positive WB detected inC6 cells, HEK-293 cells, HL-60 cells, Jurkat cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

The immunogen of 20107-1-AP is EEF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF2 fusion protein Ag13826
Full Name eukaryotic translation elongation factor 2
Calculated molecular weight 858 aa, 95 kDa
Observed molecular weight 95 kDa, 100 kDa
GenBank accession numberBC126259
Gene symbol EEF2
Gene ID (NCBI) 1938
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EEF2 antibody 20107-1-APDownload protocol
IHC protocol for EEF2 antibody 20107-1-APDownload protocol
IF protocol for EEF2 antibody 20107-1-APDownload protocol
IP protocol for EEF2 antibody 20107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

RNA m6A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.

Authors - Xinyao Lin
humanWB

BMC Nephrol

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

Authors - Yang Lu
humanWB

Pathol Res Pract

miR-183-5p acts as a potential prognostic biomarker in gastric cancer and regulates cell functions by modulating EEF2.

Authors - Wang Li
humanWB

Biomed Microdevices

Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.

Authors - Hao-Peng Lin
humanWB

Cancer Lett

eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.

Authors - Cheng Ji