EEF2 Polyclonal antibody

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  WB analysis using 20107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20107-1-AP

  WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells.

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  WB analysis of HeLa using 20107-1-AP

  WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells.

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  WB analysis of NIH/3T3 using 20107-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20107-1-AP

  IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20107-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of HeLa using 20107-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 20107-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

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Tested Applications

• Positive WB detected in: C6 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Product Information

20107-1-AP targets EEF2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EEF2 fusion protein Ag13826
• Full Name: eukaryotic translation elongation factor 2
• Calculated Molecular Weight: 858 aa, 95 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC126259
• Gene Symbol: EEF2
• Gene ID (NCBI): 1938
• RRID: AB_10950401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13639
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.

Publications

Species	Application	Title
zebrafish	WB	Nature; A molecular network of conserved factors keeps ribosomes dormant in the egg; Authors - Friederike Leesch
human	WB	Cancer Lett; eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.; Authors - Cheng Ji
human	WB,CoIP,RIP	Carcinogenesis; PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis; Authors - Wen Chen
human	WB	Biomed Microdevices; Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.; Authors - Hao-Peng Lin
human	WB	Pathol Res Pract; miR-183-5p acts as a potential prognostic biomarker in gastric cancer and regulates cell functions by modulating EEF2.; Authors - Wang Li
human	WB	BMC Nephrol; Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.; Authors - Yang Lu