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EEF2 Polyclonal antibody

EEF2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20107-1-AP

Synonyms

EEF 2, EEF2, EF 2, EF2, Elongation factor 2



Tested Applications

Positive WB detected inC6 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20107-1-AP targets EEF2 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF2 fusion protein Ag13826
Full Name eukaryotic translation elongation factor 2
Calculated molecular weight 858 aa, 95 kDa
Observed molecular weight 95 kDa, 100 kDa
GenBank accession numberBC126259
Gene symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_10950401
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EEF2 antibody 20107-1-APDownload protocol
IHC protocol for EEF2 antibody 20107-1-APDownload protocol
IF protocol for EEF2 antibody 20107-1-APDownload protocol
IP protocol for EEF2 antibody 20107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Nature

A molecular network of conserved factors keeps ribosomes dormant in the egg

Authors - Friederike Leesch
humanWB

Cancer Lett

eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.

Authors - Cheng Ji
humanWB,CoIP,RIP

Carcinogenesis

PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis

Authors - Wen Chen
humanWB

Biomed Microdevices

Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.

Authors - Hao-Peng Lin
humanWB

Pathol Res Pract

miR-183-5p acts as a potential prognostic biomarker in gastric cancer and regulates cell functions by modulating EEF2.

Authors - Wang Li
humanWB

BMC Nephrol

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

Authors - Yang Lu