EEFSEC Polyclonal antibody, PBS Only

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• EEFSEC Polyclonal antibody, PBS Only (10628-1-PBS)

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  WB analysis using 10628-1-AP (same clone as 10628-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10628-1-AP (same clone as 10628-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 10628-1-AP (same clone as 10628-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

EEFSEC Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 10628-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

10628-1-PBS targets EEFSEC in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0980

  Product name: Recombinant human EEFSEC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 269-569 aa of BC007933

  Sequence: KLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTHKRMVQSP

• Full Name: eukaryotic elongation factor, selenocysteine-tRNA-specific
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC007933
• Gene Symbol: EEFSEC
• Gene ID (NCBI): 60678
• RRID: AB_2293378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P57772
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The amino acid selenocysteine (Sec) is incorporated into a small group of proteins that are involved in preventing cellular oxidative stress. The Sec-specific elongation factor eEFec delivers the aminoacylated selenocysteine-tRNA (Sec-tRNASec) to the ribosome and suppresses UGA codons that are upstream of Sec Insertion Sequence (SECIS) elements bound by SECIS binding protein 2 (SBP2)[PMID:22992746]. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon.