Ephrin B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Ephrin B1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12999-1-AP

Synonyms

CFND, CFNS, EFL 3, EFL3, EFNB1, Elk L, ELK ligand, ephrin B1, EPLG2, LERK 2, LERK2

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Validation Data Gallery

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  WB analysis using 12999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12999-1-AP (Ephrin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12999-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12999-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat kidney tissue
• Positive IHC detected in: human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12999-1-AP targets Ephrin B1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ephrin B1 fusion protein Ag3669
• Full Name: ephrin-B1
• Calculated Molecular Weight: 346 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC016649
• Gene Symbol: Ephrin B1
• Gene ID (NCBI): 1947
• RRID: AB_10644156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Signal Transduct Target Ther; Aberrant epithelial cell interaction promotes esophageal squamous-cell carcinoma development and progression; Authors - Liping Chen ; KD Validated
human,mouse,rat	WB	J Diabetes Clin Res; Ephrin B1 Regulates Inflammatory Pathways in Retinal Müller Cells; Authors - Li Liu