Tested Applications
| Positive WB detected in | rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19291-1-AP targets EGFL7 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6559 Product name: Recombinant human EGFL7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 20-273 aa of BC012377 Sequence: TEHAYRPGRRVCAVRAHGDPVSESFVQRVYQPFLTTCDGHRACSTYRTIYRTAYRRSPGLAPARPRYACCPGWKRTSGLPGACGAAICQPPCRNGGSCVQPGRCRCPAGWRGDTCQSDVDECSARRGGCPQRCINTAGSYWCQCWEGHSLSADGTLCVPKGGPPRVAPNPTGVDSAMKEEVQRLQSRVDLLEEKLQLVLAPLHSLASQALEHGLPDPGSLLVHSFQQLGRIDSLSEQISFLEEQLGSCSCKKDS Predict reactive species |
| Full Name | EGF-like-domain, multiple 7 |
| Calculated Molecular Weight | 273 aa, 30 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC012377 |
| Gene Symbol | EGFL7 |
| Gene ID (NCBI) | 51162 |
| RRID | AB_10643993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHF1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Epidermal growth factor-like domain-containing protein 7 (EGFL7), also known as vascular endothelial statin, is an endothelial cell-derived secreted factor that regulates vascular tube formation. This protein may be involved in the growth and proliferation of tumor cells. Recent studies have reported elevated expression of EGFL7 in several tumors and cancer cell lines, including kidney tumors, malignant gliomas, hepatocellular carcinomas, and colon cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EGFL7 antibody 19291-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is anti-EGFL7 (Cat# 19291-1-AP), with a total of 12 citations. Journals include Mol Cancer, Sci China Life Sci, Blood Adv, Int J Mol Med, Mol Ther Nucleic Acids, Front Pharmacol, Hum Gene Ther, Tumour Biol, Front Oncol, Biomed Rep, Eur J Histochem, and Heliyon. Research fields span oncology (renal cell carcinoma, pituitary adenomas, multiple myeloma, glioma, osteosarcoma, cholangiocarcinoma), angiogenesis, pulmonary disease, and spinal cord injury repair.
| Species | Application | Title |
|---|---|---|
Mol Cancer A positive feed-forward loop between LncRNA-URRCC and EGFL7/P-AKT/FOXO3 signaling promotes proliferation and metastasis of clear cell renal cell carcinoma.
| ||
Sci China Life Sci Role of EGFL7/EGFR-signaling pathway in migration and invasion of growth hormone-producing pituitary adenomas. | ||
Int J Mol Med Astragalus polysaccharides exert protective effects in newborn rats with bronchopulmonary dysplasia by upregulating the expression of EGFL7 in lung tissue. | ||
Blood Adv The EGFL7-ITGB3-KLF2 axis enhances survival of multiple myeloma in preclinical models.
| ||
Mol Ther Nucleic Acids Role of ANKHD1/LINC00346/ZNF655 Feedback Loop in Regulating the Glioma Angiogenesis via Staufen1-Mediated mRNA Decay. | ||
Front Pharmacol Atractylenolide III Attenuates Angiogenesis in Gastric Precancerous Lesions Through the Downregulation of Delta-Like Ligand 4. |
