Pikachurin Polyclonal antibody

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  WB analysis of human placenta using 14578-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14578-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14578-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 14578-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Pikachurin antibody (14578-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 14578-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Pikachurin antibody (14578-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Pikachurin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Cat No. 14578-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, CoIP, ELISA

Synonyms

EGFLAM, EGF-like, fibronectin type-III and laminin G-like domain-containing protein, AGRNL, Agrin-like protein, AGRINL

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Tested Applications

• Positive WB detected in: human placenta tissue, PC-3 cells, SH-SY5Y cells
• Positive IF-P detected in: mouse eye tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

14578-1-AP targets Pikachurin in WB, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5986

  Product name: Recombinant human EGFLAM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-152 aa of BC033177

  Sequence: MRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDSVDGKNINTCGAK

• Full Name: EGF-like, fibronectin type III and laminin G domains
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC033177
• Gene Symbol: Pikachurin
• Gene ID (NCBI): 133584
• RRID: AB_2277653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q63HQ2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This antibody detects a 60-kDa band, which has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Stem Cells Dev; Pikachurin Is Partially Involved in the Synaptic Connection Between Donor and Host Cells in Late-Stage rd1 Mice Following Conspecific Photoreceptor Transplantation.; Authors - Chen Liang
human	WB,CoIP	Int Immunopharmacol; EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma; Authors - Kaishu Li
mouse	IF	iScience; The prion protein is required for normal responses to light stimuli by photoreceptors and bipolar cells; Authors - James F Striebel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (07-01-2025)

This antibody works nicely on cryosections, antigen retrieval is a must (Citrate Buffer, pH 6). Does not work on paraffin-embedded tissues.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 200
• Cell Tissue Type: Adult mouse retina