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CoraLite® Plus 488-conjugated EGFR Monoclonal antibody
EGFR Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A2H10
Cat no : CL488-66455
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66455 targets EGFR in FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | EGFR fusion protein Ag24947 |
Full Name | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
Calculated Molecular Weight | 1210 aa, 134 kDa |
Observed Molecular Weight | 145-165 kDa |
GenBank Accession Number | BC094761 |
Gene Symbol | EGFR |
Gene ID (NCBI) | 1956 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 EGFR antibody CL488-66455 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |