CoraLite® Plus 488 Anti-Human EGFR Rabbit Recombinant Antibody

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  FC experiment of A431 using CL488-98344

  1x10^6 A431 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human EGFR Rabbit RecAb (CL488-98344, Clone: 242684A1) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were not fixed.

EGFR Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL488-98344

Clone No.242684A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

242684A1, EGF R, EGF Receptor , Epidermal growth factor receptor, Proto-oncogene c-ErbB-1

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Tested Applications

• Positive FC detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98344 targets EGFR in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EGFR fusion protein Eg0204
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 134kd
• GenBank Accession Number: NM_005228.5
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00533
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.