Anti-Human EGFR Rabbit scFv Antibody

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• Anti-Human EGFR Rabbit scFv Antibody (SF98344-1-RR)

EGFR Recombinant Antibody for FC

Cat No. SF98344-1-RR

Clone No.242684A1

Host / Type

Rabbit / scFv

Reactivity

human

Applications

FC

Synonyms

EGF R, EGF Receptor , Epidermal growth factor receptor, Proto-oncogene c-ErbB-1

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  FC experiment of A431 using SF98344-1-RR

  1x10^6 A431 cells (left) or SW480 cells (right) were surface stained with 0.25 ug Anti-Human EGFR Rabbit scFv Antibody (SF98344-1-RR, Clone: 242684A1) (red) or 0.25 ug Anti-N/A IgG Isotype Control Rabbit scFv Antibody (SF98136-1-RR, Clone: 240953C9) (blue), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

SF98344-1-RR targets EGFR in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Rabbit / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  CatNo: Eg0204

  Product name: Recombinant Human EGFR protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 25-645 aa of NM_005228.5

  Sequence: LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 134kd
• GenBank Accession Number: NM_005228.5
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Source: Anti-Human EGFR scFv from clone 242684A1, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 5mM DTT, 5% Trehalose, 0.01% Proclin300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.