EHD2 Recombinant antibody, PBS Only

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  WB analysis using 85604-4-RR (same clone as 85604-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85604-4-RR (EHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85604-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85604-4-RR (same clone as 85604-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85604-4-RR (EHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85604-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85604-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85604-4-RR against Human EHD2 were performed. The affinity constant is below 1 pM.

EHD2 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85604-4-PBS

Clone No.243038C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

EH domain containing 2, EH domain containing protein 2, EH domain-containing protein 2, PAST homolog 2, PAST2

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Product Information

85604-4-PBS targets EHD2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EHD2 fusion protein Ag2002
• Full Name: EH-domain containing 2
• Calculated Molecular Weight: 543 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC014445
• Gene Symbol: EHD2
• Gene ID (NCBI): 30846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZN4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EH domain-containing protein 2 (EHD2) is a member of the EHD protein family. It is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. EHD2 is also a nuclear-cytoplasmic shuttle protein, acting as a corepressor of transcription to inhibit gene transcription when it enters the cell nucleus. EHD2 is a dynamin-related ATPase that plays a key role in regulating a series of biological processes. As a crucial regulator of endocytic transport, it is involved in the formation and maintenance of endosomal tubules and vesicles, which are vital for intracellular transport of proteins and other substances. EHD2 has been implicated in several human cancers and has potential as a predictive biomarker of chemotherapy efficacy.