EHD2 Recombinant antibody

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  WB analysis using 85604-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85604-4-RR (EHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85604-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85604-4-RR (EHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85604-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85604-4-RR against Human EHD2 were performed. The affinity constant is below 1 pM.

EHD2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85604-4-RR

Clone No.243038C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EH domain containing 2, EH domain containing protein 2, EH domain-containing protein 2, PAST homolog 2, PAST2

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-231 cells, A549 cells, mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85604-4-RR targets EHD2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EHD2 fusion protein Ag2002
• Full Name: EH-domain containing 2
• Calculated Molecular Weight: 543 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC014445
• Gene Symbol: EHD2
• Gene ID (NCBI): 30846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZN4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EH domain-containing protein 2 (EHD2) is a member of the EHD protein family. It is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. EHD2 is also a nuclear-cytoplasmic shuttle protein, acting as a corepressor of transcription to inhibit gene transcription when it enters the cell nucleus. EHD2 is a dynamin-related ATPase that plays a key role in regulating a series of biological processes. As a crucial regulator of endocytic transport, it is involved in the formation and maintenance of endosomal tubules and vesicles, which are vital for intracellular transport of proteins and other substances. EHD2 has been implicated in several human cancers and has potential as a predictive biomarker of chemotherapy efficacy.