EHD4 Polyclonal antibody

EHD4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 11382-2-AP

Synonyms

HCA11, HCA10, FKSG7, EH domain-containing protein 4, EH domain containing protein 4

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Validation Data Gallery

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  WB analysis using 11382-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11382-2-AP (EHD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11382-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11382-2-AP (EHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11382-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11382-2-AP (EHD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 11382-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11382-2-AP (EHD4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 11382-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11382-2-AP (EHD4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 11382-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11382-2-AP (EHD4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11382-2-AP

  Immunofluorescent analysis of HepG2 cells, using EHD4 antibody 11382-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A431 cells, mouse testis tissue, HeLa cells, HEK-293T cells, HepG2 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

11382-2-AP targets EHD4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EHD4 fusion protein Ag1942
• Full Name: EH-domain containing 4
• Calculated Molecular Weight: 541 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC006287
• Gene Symbol: EHD4
• Gene ID (NCBI): 30844
• RRID: AB_2277764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

H-domain containing 4, also known as EHD4, belongs to the EHD protein family. Northern blot analysis detected expression of 4 mRNA species of approximately 2.0, 2.7, 3.2, and 3.6 kb. All of the transcripts are highly expressed in pancreas, and the 2.7- and 3.6-kb transcripts are highly expressed in heart. Only weak expression is found in other tissues.

Publications

Species	Application	Title
human	WB	EBioMedicine; EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure.; Authors - Quan Zhou
mouse	WB,IHC	J Am Soc Nephrol; Reduced Glomerular Filtration in Diabetes is Attributable to Loss of Density and Increased Resistance of Glomerular Endothelial Cell Fenestrations.; Authors - Natalie Finch
mouse	WB	Curr Biol; EHD Proteins Cooperate to Generate Caveolar Clusters and to Maintain Caveolae during Repeated Mechanical Stress.; Authors - Ivana Yeow
mouse	WB	Life Sci; EHD3 positively regulated by NR5A1 participates in testosterone synthesis via endocytosis.; Authors - Lingling Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

I works well on HEK293T for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T